Analytical liquid chromatographic (LC) methods may be designed to combine reproducible and highly resolving separation with accurate and precise detection. Herein, a prototype size exclusion chromatographic (SEC) column is described and tested for various AAV serotypes. Its pore size, inert particle chemistry, particle size and bio-inert hardware were found to enable highly reproducible separation and recoveries of the AAV monomer and its aggregates and impurities even amongst batches of columns. The percentage relative standard deviations of the peak area, retention time, and % monomer were all < 1%. This performance not only enables the determination of the critical quality attributes in drug substances and drug products but also allows the use of SEC to develop ion exchange and reverse phase LC methods.